Bacterial Cellulose Synthesis by Gluconacetobacter xylinus: Enhancement via Fed-batch Fermentation Strategies in Glycerol Media

Bacterial cellulose (BC) is an abundant polysaccharide, which secreted by several genera of bacteria. It has remarkable characteristics, include high purity, tensile strength, biocompatibility and non-toxic. The main feature that differentiates BC plant (PC) the absence contaminants such as lignin, hemicellulose pectin. However, drawbacks in producing are low yield expensive carbon source. Due to that, this study was carried out enhance volumetric productivity fed-batch operation mode using glycerol a produced fill-and-draw pulse-feed cultures Gluconacetobacter xylinus DSM 46604 3-L bench-top bioreactor. fermentation trials were conducted agitated aerobic conditions at 30 ºC. For culture, total 24.2 g/L accumulated bioreactor after 9 days, corresponded 0.2 g/g 2.69 g/L/day, respectively. Pulse-feed resulted 0.38 2.71 culture proved be better system for utilizing glycerol, low-cost source.
 HIGHLIGHTS
 
 Komagataeibacter species, formerly known Acetobacter or one Gram-negative producers secretes large quantity microfibrils extracellularly
 One challenges bacterial production processing cost
 As modes bioprocess can control microbial growth rate, BC, substrate consumption also productivity
 Fill-and-draw pulse feed productivity. favorable source
 GRAPHICAL ABSTRACT